Single-Molecule Total Internal Reflection Fluorescence Microscopy
نویسندگان
چکیده
منابع مشابه
* Single - molecule fluorescence imaging by total internal reflection fluorescence microscopy ( IUPAC Technical Report )
Total internal reflection fluorescence (TIRF) is a popular illumination technique in microscopy, with many applications in cell and molecular biology and biophysics. The chief advantage of the technique is the high contrast that can be achieved by restricting fluorescent excitation to a thin layer. We summarise the optical theory needed to understand the technique and various aspects required f...
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20 We demonstrate a simple method for combining instant structured illumination microscopy (SIM) 21 with total internal reflection fluorescence microscopy (TIRF), doubling the spatial resolution of TIRF 22 (down to 115 +/13 nm) and enabling imaging frame rates up to 100 Hz over hundreds of time points. 23 We apply instant TIRF-SIM to multiple live samples, achieving rapid, high contrast super-r...
متن کاملTotal internal reflection fluorescence (TIRF) microscopy.
Total internal reflection fluorescence (TIRF) microscopy (TIRFM) is an elegant optical technique that provides for the excitation of fluorophores in an extremely thin axial region ("optical section"). The method is based on the principle that when excitation light is totally internally reflected in a transparent solid (e.g., coverglass) at its interface with liquid, an electromagnetic field, ca...
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The goal in fluorescence microscopy is to detect the signal of fluorescently labeled molecules with great sensitivity and minimal background noise. In epifluorescence microscopy, it is difficult to observe weak signals along the optical axis, owing to the overpowering signal from the out-of-focus particles. Confocal microscopy uses a small pinhole to produce thin optical sections ( 500 nm), but...
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Marvelous background rejection in total internal reflection fluorescence microscopy (TIR-FM) has made it possible to visualize single-fluorophores in living cells. Cell signaling proteins including peptide hormones, membrane receptors, small G proteins, cytoplasmic kinases as well as small signaling compounds have been conjugated with single chemical fluorophore or tagged with green fluorescent...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2016
ISSN: 1940-3402,1559-6095
DOI: 10.1101/pdb.top077800